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vector immpress duet immunohistochemistry kit  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vector immpress duet immunohistochemistry kit
    Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. <t>Immunohistochemistry</t> showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.
    Vector Immpress Duet Immunohistochemistry Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector immpress duet immunohistochemistry kit/product/Vector Laboratories
    Average 94 stars, based on 45 article reviews
    vector immpress duet immunohistochemistry kit - by Bioz Stars, 2026-06
    94/100 stars

    Images

    1) Product Images from "A humanized mouse model for adeno-associated viral gene therapy"

    Article Title: A humanized mouse model for adeno-associated viral gene therapy

    Journal: Nature Communications

    doi: 10.1038/s41467-024-46017-0

    Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. Immunohistochemistry showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.
    Figure Legend Snippet: Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. Immunohistochemistry showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.

    Techniques Used: Injection, Expressing, Immunohistochemistry, Staining



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    Vector Laboratories vector immpress duet immunohistochemistry kit
    Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. <t>Immunohistochemistry</t> showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.
    Vector Immpress Duet Immunohistochemistry Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector immpress duet immunohistochemistry kit/product/Vector Laboratories
    Average 94 stars, based on 1 article reviews
    vector immpress duet immunohistochemistry kit - by Bioz Stars, 2026-06
    94/100 stars
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    Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. Immunohistochemistry showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.

    Journal: Nature Communications

    Article Title: A humanized mouse model for adeno-associated viral gene therapy

    doi: 10.1038/s41467-024-46017-0

    Figure Lengend Snippet: Humanized TIRF and TIRFA mice were injected (1 × 10 12 gc/mouse) with AAV8 ( a , b ) or AAV9 ( c , d ) expressing tdTomato and harvested after a month. Immunohistochemistry showing co-staining of hLDH (magenta) and tdTomato (brown) in livers of humanized TIRF and TIRFA mice (low and high repopulated). Quantification of human hepatocytes expressing tdTomato from mice injected with ( b ) AAV8 ( n = 6 and n = 9 lobes for humanized TIRF and TIRFA, respectively) or ( d ) AAV9 ( n = 5 lobes for both humanized TIRF and TIRFA). hLDH human lactate dehydrogenase. Data are presented as means ± SD. Significance was validated with a two-sided student t -test. *** P ≤ 0.001 and **** P ≤ 0.0001. Source data are provided as a Source Data file. Scale bars represent 100 μm.

    Article Snippet: Immunofluorescence (IF) was developed using fluorescent-labeled secondary antibodies (Jackson ImmunoResearch Laboratories) and IHC was developed following Vector ImmPRESS Duet immunohistochemistry kit manufacturer’s instructions (Vector Labs, cat# MP-7714–15).

    Techniques: Injection, Expressing, Immunohistochemistry, Staining